Anti-phospho DCC (Tyr1418)

Application

Research CategoryApoptosis & Cancer

Anti-phospho DCC (Tyr1418), Cat. No. ABC1466, iis a rabit polyclonal antibody that detects Netrin receptor DCC and has been tested for use in Dot Blot, Immunofluorescence, and Western Blotting.

Dot Blot Analysis: A representative lot detected phospho DCC (Tyr1418) in Dot Blot applications (Antoine-Bertrand, J., et. al. (2016). J Biol Chem. 291(9):4589-602).

Immunofluorescence Analysis: A representative lot detected phospho DCC (Tyr1418) in Immunofluorescence applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385).

Western Blotting Analysis: A representative lot detected phospho DCC (Tyr1418) in Western Blotting applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385; Petrie, R.J., et. al. (2009). Biol Cell. 101(2):77-90).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Netrin receptor DCC (UniProt: Q63155; also known as Tumor suppressor protein DCC, Deleted in Colorectal Cancer) is encoded by the Dcc gene (Gene ID: 25311) in rat. Netrin receptor DCC is a single-pass type I membrane protein that serves as a receptor for netrin and is essential for axon guidance. In response to netrin-1 stimulation, DCC becomes a signaling platform to recruit proteins that promote axon outgrowth and guidance. It is detected in embryonic spinal cord, predominantly in axons of commissural neurons. Deficiencies in netrin-1 or DCC expression can result in the loss of cerebral and spinal commissures. Netrin receptor DCC also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand and has been implicated as a tumor suppressor gene. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off. It has four Ig-like C2-type domains and six fibronectin type-III domains. In response to netrin-1 stimulation DCC is phosphorylated on serine, threonine, and tyrosine residues. Its phosphorylation on Tyrosine 1418 by Src family kinases is shown to be essential for netrin-1 to mediate axon outgrowth and guidance. It has been reported that in response to netrin-1, p120RasGAP is recruited to DCC in growth cones and forms a multiprotein complex with focal adhesion kinase (FAK) and extracellular signal regulated kinase (ERK). Activities of both Ras and ERK as shown to be elevated in p120RasGAP deficient neurons. (Ref.: Antoine-Bertrand J., et al., (2016). J. Biol. Chem. 291(9):4589-602).

Immunogen

A linear peptide corresponding to 15 amino acids surrounding phospho-tyrosine 1418 from the C-terminal region of rat Netrin receptor DCC.

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.

Unpurified

Quality

Evaluated by Western Blotting in HEK293 transfected with pRK5-DCC (DCCWT).

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho DCC (Tyr1418) in HEK293 transfected wtih pRK5-DCC (DCCWT).

Specificity

This rabbit polyclonal antibody detects Netrin receptor DCC phosphorylalted on Tyrosine 1418 in rat cells. It targets an epitope within 15 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~190 kDa observed; 157.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).